{Also, a major emphasis in the event of the software package package was the emphasis on comprehensive empirical validation, and in excess of 1300 exclusive primer pairs are actually productively built and screened, with over ninety four% of these producing amplicons from the anticipated dimension, and an average mapping efficiency of ninety three% when screened utilizing bisulfite multiplex resequencing. The prospective use in the software package in other bisulfite-centered purposes which include methylation-unique PCR is under consideration for long term updates. This resource is freely obtainable for use at PrimerSuite Web-site ().
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A simple system for estimating global DNA methylation employing bisulfite PCR of repetitive DNA features
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Therapy of PCR goods with exonuclease I and heat-labile alkaline phosphatase increases the visibility of blended bisulfite restriction Investigation
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